Composite

Part:BBa_K238002:Design

Designed by: Annelien Verfaillie   Group: iGEM09_KULeuven   (2009-07-15)


Blue light promoter and key


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 215
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 92


Design Notes

restriction sites in the sequence needed to be checked.


Source

PCR-ing of a sequence to get the promoter key is from the registry

References